Methods for analysis of biomolecules are introduced. Possibilities, limitations and fields of application are discussed.
Students unterstand the theoretical basis of the essential methods in instrumental analytics for biomolecules such as proteins, nucleic acids, lipids and saccharides. They know the areas of application and are able to assess the advantages as well as the limitations of these methods.
- Spectrophotometry: Design photometer, methods for measuring adsorption, enzyme measurements, kinetics;
- Fluorimetry and luminescence: Fluorophors, design fluorimeter, measuring fluorescence, examples;
- IR- and ramanspectroscopy: Basics, Design IR spectrometer, examples;
- Liquid chromatography (HPLC): Basics, Design of HPLC equipment, column technology, methods for detection, examples (mainly RP-HPLC);
- Elektrophoresis: Basics, Free-flow, SDS-PAGE, IEF, Capillary elpho, examples;
- Mass spektrometry: general principles, mass selection, Detectors: Sector field, MALDI, MS-MS, TOF, coupling techniques, proteom techniques;
- NMR spektroscopie; Introduction to NMR, basics of NMR spectrometer equipment; chemical shift, 1H, 13C, 15N-NMR, examples;
- Miniaturization: DNA-, RNA-, protein chips, Lab-on-a-chip, nanotechnology.
Duration in Semester
6 CrP; an estimated 180 hours, of which approximately 75 are spent in class.
Weekly School Hours
Method of Instruction
Lecture 3 SWS, Exercises 1 SWS
Requirements for the awarding of Credit Points
Examination prerequisite: Acceptance of exercises, presentation of results
Examination: Written exam
according to examination regulations (§ 9)
- Lottspeich & Engels: Bioanalytik, Springer-Spektrum-Verlag
- Skoog & Leary: Instrumentelle Analytik, Springer
Prerequisite for Modules