Publications

  1. Marichal-Gallardoa P, Pieler M, Wolff M, Reichl U (2017) Chromatographic purification of cell culture-derived influenza A virus particles using regenerated cellulose membranes and polyethylene glycol. J Chromatogr A 1483 (3):110-119.
  2. Pieler MM, Heyse A, Wolff MW, Reichl U (2017) Specific ion effects on the particle size distributions of cell culture-derived influenza A virus particles within the Hofmeister series. Eng Life Sci DOI:10.1002/elsc.201600153.
  3. Pieler MM, Frentzel S, Bruder D, Wolff MW, Reichl U (2016) A cell culture-derived whole virus influenza A vaccine based on magnetic sulfated cellulose particles confers protection in mice against lethal influenza A virus infection. Vaccine 34 (50):6367-6374.
  4. Weigel T, Solomaier T, Wehmeyer S, Peuker A, Wolff MW, Reichl U (2016) A membrane-based purification process for cell culture-derived influenza A virus. J Biotechnol 220:12-20.
  5. Wang W, Voigt A, Wolff MW, Reichl U, Sundmacher K (2016) Binding kinetics and multi-bond: Finding correlations by synthesizing interactions between ligand-coated bionanoparticles and receptor surfaces. Anal Biochem 505:8-17.
  6. Meininger M, Stepath M, Hennig R, Cajic S, Rapp E, Rotering H, Wolff MW, Reichl U (2016) Sialic acid-specific affinity chromatography for the separation of erythropoietin glycoforms using serotonin as a ligand. Journal of chromatography B, Analytical technologies in the biomedical and life sciences 1012-1013:193-203.
  7. Serve A, Pieler MM, Benndorf D, Rapp E, Wolff MW, Reichl U (2015) Comparison of Influenza Virus Particle Purification Using Magnetic Sulfated Cellulose Particles with an Established Centrifugation Method for Analytics. Anal Chem 87 (21):10708-10711.
  8. Wolff MW, Fortuna AR, Pieler M, Weigel T, Fischer L, Reichl U (2014) Upcoming Chromatography Media for Cell Culture-Derived Influenza Virus Purification. Chem Ing Tech 86 (9):1486-1486.
  9. Weigel T, Solomaier T, Peuker A, Pathapati T, Wolff MW, Reichl U (2014) A flow-through chromatography process for influenza A and B virus purification. J Virol Methods 207:45-53.
  10. Wang W, Wolff MW, Reichl U, Sundmacher K (2014) Avidity of influenza virus: Model-based identification of adsorption kinetics from surface plasmon resonance experiments. J Chromatogr A 1326:125-129.
  11. Wang W, Voigt A, Wolff M, Reichl U, Sundmacher K (2014) Adsorption in einer Affinitätsmembran: Ein Ansatz mittels synthetischer Biologie. Chem Ing Tech 86 (9):1410-1410.
  12. Pieler MM, Fortuna AR, Reichl U, Wolff MW (2014) Investigation of Virus Aggregation on Performance of Downstream Processing in Influenza Vaccine Manufacturing. Chem Ing Tech 86 (9):1588-1588.
  13. Fortuna AR, Pieler M, Villain L, Reichl U, Wolff M (2014) Upcoming Membrane Supports for Influenza Virus Purification. Chem Ing Tech 86 (9):1501-1501.
  14. Neumann A, Hoyer W, Wolff MW, Reichl U, Pfitzner A, Roth B (2013) New method for density determination of nanoparticles using a CPS disc centrifuge™. Colloids Surf B Biointerfaces 104 (0):27-31.
  15. Krober T, Wolff MW, Hundt B, Seidel-Morgenstern A, Reichl U (2013) Continuous purification of influenza virus using simulated moving bed chromatography. J Chromatogr A 1307:99-110.
  16. Wolff MW, Reichl U (2011) Downstream processing of cell culture-derived virus particles. Expert Review of Vaccines 10 (10):1451-1475.
  17. Wolff MW, Siewert C, Post Hansen S, Faber R, Reichl U (2010) Purification of cell culture-derived modified vaccinia ankara virus by pseudo-affinity membrane adsorbers and hydrophobic interaction chromatography. Biotechnol Bioeng 107 (2):312-320.
  18. Wolff MW, Siewert C, Lehmann S, Post Hansen S, Djurup R, Faber R, Reichl U (2010) Capturing of cell culture-derived modified Vaccinia Ankara virus by ion exchange and pseudo-affinity membrane adsorbers. Biotechnol Bioeng 105 (4):761-769.
  19. Janke R, Genzel Y, Freund S, Wolff MW, Grammel H, Ruehmkorf C, Seidemann J, Wahl A, Reichl U (2010) Expression, purification, and characterization of a His6-tagged glycerokinase from Pichia farinosa for enzymatic cycling assays in mammalian cells. J Biotechnol 150 (3):396-403.
  20. Opitz L, Lehmann S, Reichl U, Wolff MW (2009) Sulfated membrane adsorbers for economic pseudo-affinity capture of influenza virus particles. Biotechnol Bioeng 103 (6):1144-1154.
  21. Opitz L, Hohlweg J, Reichl U, Wolff MW (2009) Purification of cell culture-derived influenza virus A/Puerto Rico/8/34 by membrane-based immobilized metal affinity chromatography. J Virol Methods 161 (2):312-316.
  22. Kröber T, Wolff MW, Lehmann S, Zimmermann A, Reichl U (2012) Accounting for the entire influenza Hemagglutinin during a flu vaccine production process. In: Jenkins N, Niall B, Alves PM (eds) European society for animal cell technology (ESACT), Dublin, Ireland, 2009. Springer,
  23. Wolff MW, Reichl U (2008) Downstream processing: From egg to cell culture-derived influenza virus particles. Chemical Engineering & Technology 31 (6):846-857.
  24. Opitz L, Zimmermann A, Lehmann S, Genzel Y, Lubben H, Reichl U, Wolff MW (2008) Capture of cell culture-derived influenza virus by lectins: strain independent, but host cell dependent. J Virol Methods 154 (1-2):61-68.
  25. Opitz L, Salaklang J, Buttner H, Reichl U, Wolff MW (2007) Lectin-affinity chromatography for downstream processing of MDCK cell culture derived human influenza A viruses. Vaccine 25 (5):939-947.
  26. Opitz L, Lehmann S, Zimmermann A, Reichl U, Wolff MW (2007) Impact of adsorbents selection on capture efficiency of cell culture derived human influenza viruses. J Biotechnol 131 (3):309-317.
  27. Kalbfuss B, Wolff M, Morenweiser R, Reichl U (2007) Purification of cell culture-derived human influenza A virus by size-exclusion and anion-exchange chromatography. Biotechnol Bioeng 96 (5):932-944.
  28. Kalbfuss B, Wolff M, Geisler L, Tappe A, Wickramasinghe R, Thom V, Reichl U (2007) Direct capture of influenza A virus from cell culture supernatant with Sartobind anion-exchange membrane adsorbers. J Membr Sci 299 (1-2):251-260.
  29. Kalbfuss B, Genzel Y, Wolff M, Zimmermann A, Morenweiser R, Reichl U (2007) Harvesting and concentration of human influenza A virus produced in serum-free mammalian cell culture for the production of vaccines. Biotechnol Bioeng 97 (1):73-85.
  30. Bandaru KSVSR, Kessler LC, Wolff MW, Reichl U, Seidel-Morgenstern A, Pushpavanam S (2007) Hydrodynamic characteristics and expansion behavior of beds containing single and binary mixtures of particles. Industrial & Engineering Chemistry Research 46 (13):4686-4694.
  31. Fehr M, Lalonde S, Lager I, Wolff MW, Frommer WB (2003) In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors. J Biol Chem 278 (21):19127-19133.
  32. Wolff MW, Su TZ (2002) Cloning and tissue-specific expression of spliced variants of the rat organic anion transporter (rOAT-K). DNA Seq 13 (1):15-25.
  33. Zhang F, Wolff MW, Williams D, Busch K, Lang SC, Murhammer DW, Linhardt RJ (2001) Affinity purification of secreted alkaline phosphatase produced by baculovirus expression vector system. Appl Biochem Biotechnol 90 (2):125-136.
  34. Wolff MW, Zhang F, Roberg JJ, Caldwell EE, Kaul PR, Serrahn JN, Murhammer DW, Linhardt RJ, Weiler JM (2001) Expression of C1 esterase inhibitor by the baculovirus expression vector system: preparation, purification, and characterization. Protein Expr Purif 22 (3):414-421.
  35. Wolff MW, Gong H, Su TZ (2000) Multiplicity of organic anion transporters by alternative splicing in rat kidney. FASEB J 14 (8):A1361-A1361.
  36. Wang Q, Wolff M, Polat T, Du Y, Linhardt RJ (2000) Inhibition of neuraminidase with neuraminic acid C-glycosides. Bioorg Med Chem Lett 10 (9):941-944.
  37. Choe J, Zhang F, Wolff MW, Murhammer DW, Linhardt RJ, S. Dordick JS (2000) Separation of a-acid glycoprotein glycoforms using affinity-based reversed micellar extraction and separation. Biotechnol Bioeng 70 (5):484-490.
  38. Wolff MW, Murhammer DW, Linhardt RJ (1999) Release and preparation of intact and unreduced N-linked oligosaccharides from SF-9 insect cells. Prep Biochem Biotechnol 29 (1):1-21.
  39. Wolff MW, Murhammer DW, Jarvis DL, Linhardt RJ (1999) Electrophoretic analysis of glycoprotein glycans produced by lepidopteran insect cells infected with an immediate early recombinant baculovirus encoding mammalian beta1,4-galactosyltransferase. Glycoconj J 16 (12):753-756.
  40. Wolff MW, Bazin HG, Lindhardt RJ (1999) Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry. Biotechnol Tech 13 (11):797-801.
  41. Bazin HG, Wolff MW, Linhardt RJ (1999) Regio- and Stereoselective Synthesis of beta-D-Gluco-, alpha-L-Ido-, and alpha-L-Altropyranosiduronic Acids from Delta(4)-Uronates. J Org Chem 64 (1):144-152.
  42. Caldwell EEO, Wolff MW, Kaul P, Serrahn JN, Murhammer DW, Weiler JM (1998) Baculovirus expression of human complement C1 esterase inhibitor. Mol Immunol 35 (6-7):34.