Current research projects

Development of downstream processes for nanoplexes

• Cell culture derived viral vectors
  • Orf-virus
  • Adeno-associated viruses
• Cell culture derived oncolytic viruses.
• Development of chromatographic methods for the purification of nanoplexes
  • Steric exclusion chromatography
  • Restricted access media

• Development of vaccine formulations

Fig.1: Process overview for the production of Orf virus in mammalian cells¹

Projects funded by:

• Federal Minister for Economic Affairs and Energy (BMWi)

• Hessian Ministry for Science and the Arts (HMWK)
• Industry Partners

Development of formulations of biological insecticides

• Production of viral insecticides in insect cell cultures to control Drosophila suzukii.

Projects funded by:

• Hessian Ministry for Science and the Arts (HMWK)
• Federal Ministry of Food and Agriculture (BMEL)

Microfluidic approach for the quantification of infectious virus particles

• Quantification of a cell culture derived baculovirus (model virus) as well as pharmaceutically relevant viral candidates

Fig.2: Analytical methods for the quantification of viruses and virus-like particles.

Project funded by:

• Federal Ministry for Economic Affairs and Climate Action (BMWK) via the German Federation of Industrial Research Associations (AiF)
  

Development of a platform technology for the production of recombinant antimicrobial proteins and peptides

References:

1 K. Lothert, F. Pagallies, F. Eilts, A. Sivanesapillai, M. Hardt, A. Moebus, T. Feger, R. Amann, M.W. Wolff, A scalable downstream process for the purification of the cell culture-derived Orf virus for human or veterinary applications, J. Biotechnol., 323 (2020) 221-230.