Module Code
Module Coordinators
Jürgen Hemberger
  • Franz Cemic
  • Jürgen Hemberger
  • Harald Andreas Lange
  • Short Description

    Spectroscopy: UV/VIS, IR/Raman, fluorometry. Chromatography: HPLC, GC. Electrophoresis: Free-flow, SDS-PAGE, IEF, CE. Mass Spectrometry: MALDI-TOF, ESI, EI, quadrupoles, ion traps. Practical applications. Students will be able to describe the functions of analytical measuring devices, will understand physical measuring principles, assess and explain analytical methods, and competently select appropriate methods.

    Learning Objectives
    Students unterstand the theoretical basis of the essential methods in instrumental analytics for biomolecules such as proteins, nucleic acids, lipids and saccharides. They know the areas of application and are able to assess the advantages as well as the limitations of these methods.
    • Spectrophotometry: Design photometer, methods for measuring adsorption, enzyme measurements, kinetics;
    • Fluorimetry and luminescence: Fluorophors, design fluorimeter, measuring fluorescence, examples;
    • IR- and ramanspectroscopy: Basics, Design IR spectrometer, examples;
    • Liquid chromatography (HPLC): Basics, Design of HPLC equipment, column technology, methods for detection, examples (mainly RP-HPLC);
    • Elektrophoresis: Basics, Free-flow, SDS-PAGE, IEF, Capillary elpho, examples;
    • Mass spektrometry: general principles, mass selection, Detectors: Sector field, MALDI, MS-MS, TOF, coupling techniques, proteom techniques;
    • NMR spektroscopie; Introduction to NMR, basics of NMR spectrometer equipment; chemical shift, 1H, 13C, 15N-NMR, examples;
    • Miniaturization: DNA-, RNA-, protein chips, Lab-on-a-chip, nanotechnology.
    Duration in Semester
    Instruction Language
    Total Effort
    6 CrP; an estimated 180 hours, of which approximately 75 are spent in class.
    Weekly School Hours
    Method of Instruction

    Lecture 3 SWS, Exercises 1 SWS

    Requirements for the awarding of Credit Points

    Examination prerequisite: Acceptance of exercises, presentation of results

    Examination: Written exam

    Evaluation Standard
    according to examination regulations (§ 9)
    • Lottspeich & Engels: Bioanalytik, Springer-Spektrum-Verlag
    • Skoog & Leary: Instrumentelle Analytik, Springer
    Prerequisite for Modules